Laboratories IKBFU

Laboratory of Biotechnologies and Biomedicine

General information

Our major thrust of current research concerns molecular mechanisms underlying neural and glial cell heterogeneity in the brain, the origins of this heterogeneity and its functional implementations; neuron - astrocyte interactions; the cellular populations inhabiting adult subventricular zones of the brain, particularly astrocyte-like progenitor cells, their role in the processes of neuro- and gliogenesis under normal conditions as well as in case of acute and chronic stress and neuroinflammation.

Bi-directional communication between the brain and the immune system is well established. The immune system is considered to act as a diffuse sense organ, relying the information about variety of processes to the brain via humoral and neural routes. The brain regulate the immune responses by two major pathways: hypothalamic–pituitary–adrenal axis and sympathetic-adrenal-medullary axis. Although the connections between the brain and immune organs are well-known, the exact molecular mechanisms underlying the signalling, especially the role of glial cells and cytokine production in the brain, is still a matter of discussion. We are interested in how neuroinflammation is initiated, how it develops under acute and chronic stress conditions, which neuro- and gliotransmitter systems are involved in the process as well as how the cellular populations inhabiting adult subventricular zones are affected.

Research in our laboratory is performed using molecular, genetic and cell biology approaches, gene delivery viral vectors and state-of-the-art microscopy techniques.

Our methods/techniques:

Molecular cloning, viral vector production, stereotaxic surgery, real time PCR, mammalian cell culture, as well as fluorescenc emicros copy, immunohistochemistry, flow cytometry and fluorescence-activated cell sorting. We are focused on developing high quality viral vector facility here at IKBFU.

Equipment

Nucleofector™ 2b Device , Lonza

Sorter Becton Dickinson FACS Aria III SORP

Ion PGM™ System for Next-Generation Sequencing

C1000 Touch™ Thermal Cycler

PyroMark Q24 System

MALDI-TOF/TOF Autoflex, Bruker Daltonik

ACQUITY UPLC I-Class System

VersaDoc Model 4000, Bio-RAD

QX100, Bio-Rad

PALM MicroBeam ZEISS Microscopy

S3e™ Cell Sorter

MSC Advantage 1.2

UV-Cleaner box-1200, BioSan

Research Team
Due to largely overlapping interests we have an intensive collaboration with other laboratories within the Institute, namely Laboratory for Neurobiology and Medical Physics (Dr. Vitaliy Kasymov) as well as Laboratory of Neurophysiology (Dr. Irina Vakoluk and Dr. Valery Zhukov). Our international collaborations include Dr. Anja Teschemacher and Prof. Sergey Kasparov from the Department of Physiology, Pharmacology and Neuroscience, University of Bristol (Bristol, UK), Dr. Natalie Wielsch from Research Group Mass Spectrometry & Proteomics, Max Planck Institute for Chemical Ecology (Jena, Germany) and Dr. Natalia Kononenko from the Cluster of Excellence for Aging Research CECAD (Cologne, Germany).
Publications

Tuchina O, Dominova I, Klimaviciusa L, Filiakova N, Vasilev A, Patrushev M, Kasymov V (2016). Region- and age-dependent heterogeneity of receptors involved in calcium signaling in rat astrocytes. Opera Medica et Physiologica S1.

Dominova I, Tuchina O, Klimaviciusa L, Filiakova N, Vasilev A, Patrushev M, Kasymov V (2016). Postnatal development and age- and region-dependent diversity of glial GLAST-positive cells. ASCB Annual Meeting.

Tuchina O, Dominova I, Klimaviciusa L, Filiakova N, Vasilev A, Patrushev M, Kasymov V (2016) Regional diversity of rat brain astrocytes. 10th FENS Forum of Neuroscience.

Klimaviciusa L, Dominova I, Tuchina O, Vasilev A, Filiakova N, Shusharina N, Patrushev M, Kasymov V (2015). Regional heterogeneity of rat brain astrocytes. 2nd International Bordeaux Neurocampus/Brain Conference GliSyn.

Tuchina O, Koczan S, Harzsch S, Rybak J, Wolff G, Strausfeld NJ, Hansson BS (2015). Central projections of antennular chemosensory and mechanosensory afferents in the brain of the terrestrial hermit crab (Coenobita clypeatus; Coenobitidae, Anomura). Frontiers in Neuroanatomy 9.

Tuchina O, Groh CK, Talarico G, Müller CHG, Wielsch N, Hupfer Y, Svatoš A, Grosse-Wilde E & Hansson BS (2014). Morphology and histochemistry of the aesthetasc-associated epidermal glands in terrestrial hermit crabs of the genus Coenobita (Decapoda: Paguroidea). PLoS ONE 9(5).

Polanska MA, Tuchina O, Agricola H, Hansson BS & Harzsch S (2012).
Neuropeptide complexity in the crustacean central olfactory pathway:
immunolocalization of A-type allatostatins and RFamide- like peptides in the brain
of a terrestrial hermit crab. BMC Molecular Brain 5 (29).