Laboratories IKBFU

Main research fields of the Laboratory of Immunology and cellular biotechnology

General information
  1. Mechanisms of metabolic abnormalities in insulin resistance;

  2. Cellular and molecular genetic mechanisms regulating the activation process, differentiation and self-immune memory;

  3. Development of innovative ways of diagnosing the risk of endometriosis by polymerase chain reaction in real time;

  4. Working out scientific and technological potential to create tissue-engineered constructs based biocomposite materials and cultured cells in vitro;

  5. The study of the potential therapeutic properties of multipotent mesenchymal stromal cells of post-natal tissues of animals with a view to their application in regenerative veterinary medicine.

Equipment
  1. Multimodal microplate reader Mithras Research II,

  2. Aggregometer 490-2D optical, 2-channel, including DESTEN computer set. HRONOPAR Collagen,

  3. Microscope Akiostar Plus,

  4. SKH4 Olympus inverted microscope,

  5. CO2 Incubator MCO-15AS, Sanyo,

  6. High Water purification system Simplicity UV, Millipore,

  7. Drying ovens MOV-212F, Sanyo,

  8. Hemostasis Analyzer Coagulometer ACL 7000

  9. Automatic Biochemical Analyzer CA-180,

  10. Flow digital fluorometer EasyCyte Plus Base System, Guava Technologies,

  11. Flow cytofluorometer MACSQuant Analyzer,

  12. Automatic immunomagnetic cell sorter AutoMACS Pro starter kit,

  13. Device for biomarkers diagnosis of Bio-Plex System with the automated Bio-Plex Pro Wash Station plate washer system,

  14. SAS-1 Plus / SAS-2 230 Automated Protein Electrophoresis System, V-HELENA BioScienses Europe,

  15. Analyzer LightCycler 480-II for the PCR analysis in real time: a fuser 96-well plate modification,

  16. LightCycler 480 analyzer for PCR testing in real time: a fuser 384-well plate modification,

  17. LNP40 Generator of liquid nitrogen ,

  18. System for living cells culture monitoring Cell-lQ v.2 with accessories,

  19. Research biological fluorescent microscope,

  20. Real-time cell analyzer, assembled / RTCA xCELLigence DP bundle (complete with plates E-Plate-hole.)

  21. Automated Immunoenzymometric Analyzer "Lazurite" (Lapis) with accessories, "Dynex Technologies"

Research Team
  1. Литвинова Л.С.Litvinova Larisa, Dr. of Medicine, Head of the Laboratory, LLitvinova@kantiana.ru, larisalitvinova@yandex.ru
  2. Shupletsova Valeria, PhD in Biology, vshupletsova@mail.ru
  3. Kirienkova Elena, PhD. in Medicine, elenamed@list.ru
  4. Aksenova Natalia, PhD. in Biology, seme44kin@mail.ru
  5. Zima Anastasia, Dr. of Medicine, zima2302@gmail.ru
  6. Vasilenko Maria, mary-jean@yandex.ru
  7. Dunets Natalia, PhD in Biology, natalia.sokhonevich@gmail.com
  8. Skuratovskaya Darya, dariask@list.ru
  9. Haziahmatova Olga, PhD. in Biology, hazik36@mail.ru
  10. Yurova Christine, PhD. in Medicine, kristina_kofanova@mail.ru


Publications
  1. Changes of immune parameters during T-cells vaccination in patients with rheumatoid arthriris / Ivanova I.P., Seledzova G.V., Mamaev S.V., Litvinova L.S., Lopatnikova Yu.A., Sennikov S.V., Seledzov V.I. // Russian Journal of Immunology. – 2011. V. 5 (14). №3-4. P. 341-349.

  2. The impact of age and gender on the indicators of coagulator and vascular thrombocyte hemostasis in patients with II type diabetes mellitus / Kondratieva E.I., Suhanova G.A., Litvinova L.S., Kirienkova E.V., Kretova E.I., Zatolokln P.A. // Klinichescheskaya Laboratornaya Diagnostika – 2011. №8. P. 41-43.

  3. The impact of surgical correction of obesity (laparoscopic gastro-bypass surgery) under metabolic syndrome on the biochemical blood indicators / Kiriyenkova Y.V., Litvinova L.S., Seledzov V.I., Zalotkin P.A., Aksenova N.N. // Klinicheskaia laboratornaia diagnostika. - 2012. V. 12. P. 3-5.

  4. Eosinophil: A modern concept of the kinetics, structure, and function / Kolobovnikova Yu.V., Urazova O.I., Novitsky V.V., Litvinova L.S., Chumakova S.P. // Gematologiya i Transfusiologiya. – 2012. V. 57(1). P. 30-36.

  5. Dose-response effect of steroid hormones on the Gfi1 and U2afil4 gene expression in T lymphocytes at different stages of differentiation / Gutsol A.A.Sokhonevich N.A., Seledtsov V.I., Litvinova L.S. // Molecular Biology. - 2013. V. 47(4). P. 572–580.

  6. Dexamethasone effects on activation and proliferation of immune memory T cells / Gutsol A.A.Sokhonevich N.A., Seledtsov V.I., Litvinova L.S. // Bulletin of Experimental Biology and Medicine. - 2013. V. 155(4). P. 474-476.

  7. The Content of p53 Protein in Tumor Cells by the Action of a Selective Inhibitor of Hsp27 Chaperone in vitro / Kaigorodova E.V., Litvinova L.S., Konovalova E. V., Klimova М.V., Tashireva L.A., Nosareva O. L., Novitskiy V. V. // International Journal of Biology. - 2013. Vol. 5, №3. – P. 13-18.

  8. Features of cytokine-producing ability of peripheral blood mononuclear cells in the metabolic syndrome / Litvinova L.S.Kirienkova E.V., Gazatova N.D., Zatolokin P.A., Vasilenko М.А., Aksenova N.N., Simbirtsev A.S.  // Cytokines and inflammation. – 2013. №3. P. 62-66.

  9. Effects of steroid hormones upon Th1/Th2 cytokine production by CD45RO+ T cells / Gutsol A.A.Litvinova L.S. // Medical Immunology. – 2013. V. 15(3). P. 263-268.

  10. The influence of immunoregulatory cytokines IL-2, IL-7, and IL-15 upon activation, proliferation, and apoptosis of immune memory T-cells in vitro / Litvinova L.S., Sokhonevich N.A., Gutsol A.A., Kofanova K.A. // Cell and Tissue Biology. - 2013. V. 7(6). P. 539-544.

  11. Study of the nanostructure and cytotoxicity of ZrO2 and Al2O3 ceramic implants / Litvinova L., Shupletsova V., Leitsin V., Shusharina N., Botsman S., Kulkov S., Vasyliev R., Zubov D. // Regenerative Medicine. – 2013. V. 8(6s). P. 191–192.

  12. Human endometrial stromal cells: in vitro biological properties under conventional and low oxygen cell culture conditions / Vasyliev R., Zubov D., Rodnichenko A., Litvinova L., Korop Z., Sednev O., Kurmyshov A. // Regenerative Medicine. – 2013. V. 8 (6s). P. 176–177.

  13. Pathogenesis of Insulin Resistance in Metabolic Obesity / Litvinova L.S., Kirienkova E.V., Mazunin I.O., Vasilenko M.A., Fattakhov N.S. // Biochemistry. – 2014. V. 8(3). P. 192-202.

  14. Тhe effects of testosterone and β-estradiol on activation of lymphocytes associated with IL-2 production and expression / Gutsol A.A.Sokhonevich N.A., Kofanova K.A., Litvinova L.S. // Cell and Tissue Biology. – 2014. V. 8(6). P. 473–477.

  15. Evaluating the Number of Mitochondrial DNA Copies in Leukocytes and Adipocytes from Metabolic Syndrome Patients: Pilot Study / Mozhei O.I., Zatolokin P.A., Vasilenko M.A., Litvinova L.S., Kirienkova E.V., Mazunin I.O. // Molecular Biology. – 2014. V. 48(4). P. 589–592.

  16. Role of adiponectin and pro-inflammatory gene expression in adipose tissue chronic inflammation in women with metabolic syndrome [Electronic resource] / Litvinova L.S., Atochin D.N., Vasilenko M.A., Fattakhov N.S., Zatolokin P.A., Kirienkova E.V. // Diabetology & Metabolic Syndrome. – 2014. - Mode of access: http://www.dmsjournal.com/content/6/1/137

  17. Porosity and biocompatibility study of ceramic implants based on ZrO2 and Al2O3 / Litvinova L., Shupletsova V., Leitsin V., Vasyliev R., Zubov D., Buyakov A., Kulkov S. // Conference Collection. International Conference on Physical Mesomechanics of Multilevel Systems 2014. – 2014. V. 1623. P. 347-350.

  18. The role of nitric oxide synthases in the development of mitochondrial dysfunction in metabolic syndrome [Electronic resource]   / Litvinova L.S., Atochin D.N., Fattakhov N.S., Vasilenko M.A., Zatolokin P.A., Kirienkova E.V. // Front Physiol. – 2015. - Mode of access: http://www.frontiersin.org/Physiology/about

  19. Cytokine-Mediated Regulation of Expression of Gfi1 and U2afll4 Genes by Activated T-Cells with Various Differentiation Status In Vitro / Yurova K.A., Sokhonevich N.A., Khaziakhmatova O.G., Litvinova L.S. // Biomedical Chemistry. – 2015. V. 9(2). P. 165-172.

  20. Effects of Immunoregulatory Cytokines (IL-2, IL-7, and IL-15) on Expression of Gfi 1 and U2afl l4 Genes in T Cells at Different Stages of Differentiation / Sokhonevich N.A., Yurova K.A., Gutsol A.A., Khaziakhmatova O.G., Mazunin I.O., Litvinova L.S. // Bulletin of Experimental Biology and Medicine. - 2015. - Vol. 159, No. 2. P. 236 – 239.

  21. Dose-Dependent Effects of Dexamethasone on Functional Activity of T-Lymphocytes with Different Grades of Differentiation / Gutsol A.A., Sokhonevich N.A., Yurova K.A., Khaziakhmatova O.G., Shupletsova V.V., Litvinova L.S. // Molecular Biology. - 2015. Vol. 49, No. 1. P. 130–137.

  22. Phenotypic characterization and functional features of memory T- and B-cells / Sokhonevich N.A., Khaziakhmatova O.G., Yurova K.A., Shupletsova V.V., Litvinova L.S. // Tsitologiia. - 2015. Vol. 57 (5). P. 311-318.

  23. Insulin resistance pathogenesis in metabolic obesity / Litvinova L.S., Kirienkova E.V., Mazunin I.O., Vasilenko M.A., Fattakhov N.S.  // Biomedical Chemistry. – 2015. V. 61(1). P. 70-82.

  24. Nitric oxide and mitochondria in metabolic syndrome / Litvinova L., Atochin D., Fattakhov N., Vasilenko M., Zatolokin P., Kirienkova E. // Frontiers in Physiology, 2015. – Vol. 6. – Mode of access: http://journal.frontiersin.org/article/10.3389/fphys.2015.00020/abstract.