Functionalized Magnetic Materials for biomedicine and nanotechnology center

REC Laser Nanotechnology and Biophysics

General information

Scientists of the Laser Nanotechnology and Biophysics REC (headed by I.Samusev, Ph.D.) study photo processes associated with the laser-matter interaction, molecular spectroscopy and luminescence, as well as nano-optics and nanoplasmonics. They develop methods of producing metal nanoparticles (silver, gold, copper, and others.) by electrochemical deposition and femtosecond laser ablation in liquids and vacuum, while photon correlation spectroscopy is used to control nanoparticles shape and size.

A good part of the REC efforts is dedicated to researching the issues of photophysics at solid surfaces, nonlinear laser photoprocesses on the geometrically constrained surfaces (disperse systems, nanostructures, thin films). The REC team uses laser spectroscopy to study biomolecular properties to develop functional bioelectronics applications.

Fluorescence techniques that form the basis of research at the REC are widely used in biology - in particular, to study the structure of proteins by fluorescent tags, or probes. Fluorescence techniques have seen successful application in studying rapid reactions of electronically excited molecules. Raman spectroscopy is another research field with a great variety of possible applications. Nano- and femtosecond laser research makes it possible to investigate ultrafast processes in condensed media, on the surface, as well as to develop methods of producing metal nanoparticles. Metal nanoparticles are becoming increasingly popular with the manufacturers of ceramic and composite materials, superconductors, solar cells, biocompatible implants, filters, getters, lubricant additives, dyes and magnetic pigments, low-melting high-strength solder components. All research at the REC is carried out at the new equipment made by HORIBA (Japan), SOLAR SYSTEMS (Belarus), TETA (Russia), SHIMADZU (Japan), HAMAMATSU (Japan) and other reputable producers.

Years of research have lead to the formulation of main kinetic energy transformation principles of electronically excited molecules in condensed molecular systems under intense laser radiation.

The research team has pioneered a comprehensive study to determine the role of cooperative processes in the transformation of energy in the triplet excited luminophore molecules in solutions, polymers and silica surface.

The study of dyes and polycyclic aromatic carbohydrate molecules in micellar solutions revealed triplet-triplet energy transfer and mixed triplet-triplet annihilation for reagents separated by a layer of micelles as well as intra- and intermicellar TTA aromatic molecules in micelles in micellar solutions of dye molecules and aromatic carbohydrates.

Annihilation of singlet oxygen molecules and triplet luminophores in condensed matter has also been comprehensively investigated.

The research team's breakthrough study is the systemic analysis of intercombination transitions in luminophores under the influence of external heavy atoms in a variety of physical and chemical media.              

Flash photolysis has been used to study the effect of structuring of and relaxation processes in solvates on the luminophores excitation dynamics: the REC research team was the first to observe the effect of microstructuring of water and organic compounds on the redistribution of the probability of intramolecular transitions in acridine dyes, and a correlation between the deactivation constants of their triplet states and clathrate structuring of solutions.

The REC team has also conducted a comprehensive study of nonlinear photoprocesses with luminophores in fractal dispersed systems.

Photocorrelation spectroscopy of molecular scattering of light has been used to research anomalous diffusion of biomolecules and nanoparticles. The dependence of the diffusion characteristics of the molecular systems on the distance from the porous solid surface has been established and modelled as a result.

The study of nonlinear kinetic photoprocesses in aqueous-organic mixtures conditioned by the clathrate structuring of the solution revealed and prompted further research into the effect of nanostructuring of water-alcohol mixtures on the redistribution of the probabilities of intramolecular transitions in triplet excited molecules. The research team managed to establish a correlation between constants of triplet states deactivation and clathrate structuring of the solutions.

In 2014 the team developed techniques for obtaining different silver nanoparticles by chemical methods, electrochemical methods femtosecond laser ablation of silver and gold nanoparticles. At the REC we have successfully studied enhancement and suppression of photoluminescence of luminophores and quantum dots for moving plasmon energy in different media; the effect of plasmons on the deactivation rate, polarization and anisotropy of luminescence emission with donor-receptor energy transfer. We have managed to strengthen singlet-triplet-triplet and singlet-triplet energy transfer under the influence of surface plasmons of silver and gold nanoparticles in polymers and on the surface of nanoporous adsorbents, and researched processes of nonradiative transfer of electronic energy between quantum dots and luminophores in polymeric films of polyvinyl butyral with the generation of singlet oxygen and the combined effect of the plasmon and external heavy atoms. The team has also succeeded in building theoretical models of metabolic processes of organic molecules and quantum dots in different spin states for generation of surface plasmon resonance.

Spectral and kinetic methods have been applied to the study of electronic energy transfer between the quantum dots CdSe / CdS / ZnS and CdSe / ZnS on the surface of wide-pore silica in the absence and in the presence of silver nanoparticles produced by laser ablation.

The results of these studies have been published in over a hundred articles in key Russian and international journals, including, but not limited to: Journal of Applied Spectroscopy, Quantum Electronics, Russian Physics Journal, Optics and Spectroscopy, Journal of Atmospheric and Solar-Terrestrial Physics, Russian Journal of Physical Chemistry A, Geomagnetism and Aeronomy, Journal of Optical Technology, Proceedings of SPIE, Moscow University Physics Bulletin.

Current research of the REC

REC staff is currently conducting research in the framework of the research project "Spectral and kinetic studies of plasmon interaction of metal nanoparticles with organic molecules and quantum dots in different media" in the framework of the Federal contract from the Russian Federation Ministry of Education and Science (project design stage: 2014 - 2016).

The following fundamental and applied research initiatives will comprise the third stage of this project:

- Study of the combined influence of external heavy atoms and plasmon energy on deactivation processes of singlet and triplet states of luminophores.

- Study of the influence of the cluster size of nanoparticles and quantum dots Ag processes plasmon energy transfer between the resonant excitation of plasmons.

- Study of nonradiative transfer of electronic energy from the quantum dots to adsorbates of dye molecules and luminescent protein molecules (albumin); analysis of concentration dependences.

- Study of the effect of plasmon absorption and fluorescence amplification in biomolecules (albumin); study of the sizes of albumin molecules in liquids with FCC spectroscopy.

- Study of the IR spectra of silver hydrosol in the free state and in polymer films to clarify the mechanism of absorption in a polymer film and to identify the emerging links with the dye.

- Study of the photovoltaic effect in polymer films doped by luminophores, quantum dots and nanoparticles of silver to expand the range of optical absorption of photons and increase the rate of charge generation and transfer.

- Conducting a series of exploratory studies to increase the plasmon effect of fluorescence enhancement of various biological tissues (in vitro).

In 2015 the REC team took up a number of new research initiatives:

1) "Investigation of promising hybrid solar energy converters based on artificial perovskite quantum dots and metal nanoparticles". Within this project we will look at the photo processes taking place in the prototype hybrid photovoltaic inverter of solar energy of the visible and near infrared spectra based on perovskite filled with metal nanoparticles and semiconductor quantum dots . We will apply spectral and kinetic methods for the study of energy conversion efficiency of plasmon nanoparticles and electron excitation of quantum dots in the photocurrent generation and use new types of organometallic compounds in place of lead compounds.

2) "Development of methods to validate photodynamic physico-chemical treatment techniques " (in collaboration with the Laboratory of Pharmacology);

3) "Molecular dynamics of capture and release of sphingosine-1-phosphate apolipoprotein M of endothelial cells with endothelial dysfunction" (in collaboration with the Laboratory of Pharmacology).

Equipment
  • Spectral modular fluorometer Fluorolog-3 (HORIBA, Japan).

  • Pulsed Nd: YAG unable wavelength laser system (SOLAR LASER SYSTEM, Belarus).

  • Centaur U - Scanning AFM/Confocal/Raman/Fluorescence system for Raman/Fluorescence and AFM/Raman (TERS) imaging (NanoScan Technologies, Russia - Belarus):

  • Femtosecond laser system of visible and near infrared spectral bands TETA-25/30 (Avesta, Russia).

  • Model RF-5301 PC Spectrofluorimeter (SHIMADZU, Japan).

  • UV-3600 Spectrophotometer (SHIMADZU, Japan) for the UV / visible / IR range.

  • Photocor-FC. Modular Spectrometer for dynamic and statistical scattering light (Russia).

Research Team

  1. Илья СамусевSamusev, Ilya G., Ph.D., director of the REC; E-mail: ISamusev@kantiana.ru

  2. Bruchanov, Valery V., Dr. Hab., leading researcher of the REC, Professor at the department of Physics; E-mail: VBryukhanov@kantiana.ru

  3. Slezhkin, Vasily A., Ph.D., senior researcher (external part-time contract); E-mail:

  4. Myslitskaya, Natalia A., Ph.D., senior researcher (external part-time contract); E-mail:

  5. Tsibulnikova, Anna V., laboratory researcher (external part-time contract); E-mail:

  6. Zubin, Andrey Y., Graduate student, laboratory researcher (external part-time contract); E-mail: AZubin@kantiana.ru

  7. Tikhomirova, Nadezhda S., laboratory researcher (external part-time contract); E-mail: NSTikhomirova@kantiana.ru

  8. Borkunov, Rodion Y., laboratory researcher, Ph.D. student; E-mail: RBorkunov@kantiana.ru

  9. Tsarkov, Maxim V., laboratory researcher, Ph.D. student; E-mail: MTSarkov@kantiana.ru

  10. Stepanova, Elizaveta K., senior laboratory technician. E-mail:

Graduate students of the Institute of Physics and Technology (Physics, Optical Physics and Photonics), 6 pers..

Publications
  1. Tikhomirova N.S., Myslitskaya N.A., Samusev I.G., Bryukhanov V.V. Plasmon enhancement of electron energy transfer between quantum dots on PMMA surface. Journal of Applied Spectroscopy (2015) 82, 6, 850-858.

  2. Bryukhanov V.V., Konstantinova E.I., Borkunov R.Yu., Tsarkov M.V., Slezhkin V.A. Silver nanoparticles influence upon relaxation processes and the efficiency of dipole-dipole energy transfer between dye molecules in PMMA thin films. Quantum Electronics (2015) 45, 10, 908-913.

  3. Bryukhanov V.V., Minaev B.M., Tsibulnikova A.V., Slezhkin V.A. The effect of gold nanoparticles on exchange processes in collision complexes of triplet and singlet oxygen molecules with excited eosin molecules. Optics and Spectroscopy (2015) 119, 1, 29-38.

  4. Tsibulnikova A.V., Bryukhanov V.V., Slezhkin V.A. Enhancement of singlet-triplet energy transfer between dyes in a polymer film by surface plasmons of gold nanoparticles. Russian Physics Journal (2015) 57, 12, 1716-1724.

  5. Bryukhanov V.V., Tsibulnikova A.V., Samusev I.G., Slezhkin V.A. Effect of silver nanoparticles in singlet-singlet energy transfer dynamics of luminophores in thin films of polyvinyl alcohol. Journal of Applied Spectroscopy (2014) 81, 4, 570-576.

  6. Bryukhanov V.V., Minaev B.F., Tsibulnikova A.V., Tikhomirova N.S., Slezhkin V.A. Plasmon amplification and quenching of the fluorescence and phosphorescence of anionic and cationic dyes in various media. Journal of Optical Technology (2014) 81, 11, 625-630.

  7. Myslitskaya N.A., Samusev I.G., Bryukhanov V.V. Dipole-dipole electron excitation energy transfer in the system CdSe/ZnS quantum dot – eosin in butyral polymer matrix. Russian Physics Journal (2014) 57, 7, 920-928/

  8. Myslitskaya N.A., Khitrin A.V., Ivanov A.M., Samusev I.G., Bryukhanov V.V. Dynamics of colloid silver nanoparticles in evaporating water droplet. Russian Physics Journal (2012) 54, 22, 1280-1285.

  9. Bryukhanov V.V., Samusev I.G., Ivanov A.M., Myslitskaya N.A. Nanoparticle diffusion probing of the structure of water and aqueous organic solutions near a porous surface and in its bulk in a wide temperature interval. Russian Physics Journal (2009) 52, 2, 119-126.

  10. Myslitskaya N.A., Ivanov A.M., Samusev I.G., Bryukhanov V.V. Deactivation of Rodamine 6G triplet-excited molecules and diffusion of nanoparticles in water-alcohol solutions. Journal of Applied Spectroscopy (2009) 76, 6, 777-782.

  11. Klimenko M.V., Klimenko V.V., Bryukhanov V.V. Numerical modeling of the light ion and heat balance of the topside atmosphere in quiet geomagnetic conditions. Journal of Atmospheric and Solar-Terrestrial Physics (2008) 70, 2144-2158.

  12. Bryukhanov V.V., Samusev I.G., Ivanov A.M., Myslitskaya N.A. Anomalous diffusion of nanoparticles in water close to a porous surface. Russian Journal of Physical Chemistry A (2008) 82, 2, 258-262.

  13. Samusev I.G., Bryukhanov V.V., Ivanov A.M. Labutin I.S., Loginov B.A. Heterogeneous triplet-triplet annihilation of erythrosine and anthracene molecules on a fractal anodized aluminum surface. Journal of Applied Spectroscopy (2007) 74, 2, 230-236.

  14. Klimenko M.V., Klimenko V.V., Bryukhanov V.V. Numerical simulation of the electric field and zonal current in the Earth’s ionosphere: the dynamo field and equatorial electrojet. Geomagnetism and Aeronomy (2006) 46, 4, 457-466.

  15. Bryukhanov V.V., Samusev I.G., Ivanov A.M. Anomalous diffusion accompanying triplet-triplet energy transport between luminophors at a solid-liquid boundary. Journal of Optical Technology (2005) 72, 12, 900-904.

  16. Bryukhanov V.V., Ivanov A.M., Samusev I.G. Hetero-annihilation of the excited states of associates and monomers of fluorescein dyes on the silica surface at low temperatures. Journal of Applied Spectroscopy (2005) 72, 6, 804-808.

  17. Bryukhanov V.V., Samusev I.G., Karstina S.G. Effect of temperature on the rate of triplet-triplet annihilation of 1,2-benzanthracene in a polymer matrix. Journal of APplied Spectroscopy (2004) 71, 1, 54-59.

  18. Karstina S.G., Markova M.P., Bryukhanov V.V. Homo- and hetero-annihilation of triplet-excited Bengali-rose and anthracene molecules on silica in a wide temperature range. Journal of Applied Spectroscopy (2003) 70, 5, 733-739.

  19. Bryukhanov V.V., Elistratova S.A., Laurinas V.C. Spectral-luminescent properties of deposited rhodamine 6G films. Journal of Applied Spectroscopy (2003) 70, 5, 733-739.

  20. Bryukhanov V.V., Samoussev I.G. Dynamics of exchange-resonance photoprocesses on the surface of chemically modified silica. Proceedings of SPIE (2002) 4749, 336-342.

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